2-O-α-D-glucosyl glycerol production by whole-cell biocatalyst of ...

4 days ago · The food grade strain Lb. paracasei was used for the first time as cell factory to recombinantly express the LrSP and construct a whole-cell biocatalyst for the production of 2 …

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Efficient 2-O-α-D-glucopyranosyl-sn-glycerol Production By Single …

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The maximum production of 2-αGG by these combined strategies reached 351.8 g·L-1 with 98% conversion rate from 1.4 M sucrose and 3.5 M glycerol in a 5-L bioreactor. This was one of the …

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FAQs about 2-O-α-D-glucosyl glycerol production by whole-cell biocatalyst of ... Coupon?

How is glycerol produced?

Due to its natural scarcity, GG must be prepared through dedicated synthesis, and an industrial bioprocess for GG production has been implemented. This process uses sucrose phosphorylase (SucP)-catalyzed glycosylation of glycerol from sucrose, applying the isolated enzyme in immobilized form. ...

How is glycerol produced from sucrose phosphorylase (sucp)?

This process uses sucrose phosphorylase (SucP)-catalyzed glycosylation of glycerol from sucrose, applying the isolated enzyme in immobilized form. A whole cell-based enzyme formulation might constitute an advanced catalyst for GG production. ...

How effective is glycerol biosynthesis of 2GG?

The maximum production of 2-αGG by these combined strategies reached 351.8 g·L -1 with 98% conversion rate from 1.4 M sucrose and 3.5 M glycerol in a 5-L bioreactor. This was one of the best performance reported in single-cell biosynthesis of 2-αGG, which paved effective ways for industrial-scale preparation of 2-αGG. 1. Introduction ...

Is glucosylglycerol a natural osmolyte?

Glucosylglycerol (2- O -α- d -glucosyl- sn -glycerol; GG) is a natural osmolyte from bacteria and plants. It has promising applications as cosmetic and food-and-feed ingredient. Due to its natural scarcity, GG must be prepared through dedicated synthesis, and an industrial bioprocess for GG production has been implemented. ...

Can a whole cell catalyst produce osmolyte 2 O glucosylglycerol?

Whole cell-based catalyst for enzymatic production of the osmolyte 2- O -α-glucosylglycerol. Microbial Cell Factories, 20, 79. This work was financed by the National Key R & D Program of China (2021YFC2101500), NSFC (21878155), the Jiangsu Synergetic Innovation Center, or Advanced Bio-manufacture, and PAPD. J.P.L. conducted most of the experiments. ...

What is 2-O-D-glucosyl glycerol used for?

1 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China. 2-O-α-D-Glucosyl glycerol (2-αGG) can be used as a multipurpose anti-aging, cell-stimulating, and skin moisturizing agent in the cosmetic industry. Sucrose phosphorylase (SPase) has been widely used in the production of 2-αGG. ...